Abstract
Spectrophotometric DPPH method was used for the determination
of DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity of Scenedesmus sp. which was isolated from
the Giresun mine and cultivated under BG11 culture conditions. 1 mL of 10-3 M
DPPH ethanol solution was added and 3 mL of extract solution was added and
vortexed. The absorbance was read at 517 nm for 30 minutes in the dark. BHT and
Trolox were used as standard antioxidants. The DPPH radical scavenging activity
reaction was calculated using the formula.