Abstract
Memory impairment is a cognitive dysfunction caused by oxidative stress due to increased free radicals in brain tissue. Avocado leaves (Persea americana Mill.) are known to contain flavonoid compounds that have the potential as antioxidants and have neuroprotective effects. This study aims to determine the effect of variations in avocado leaf extraction solvents on improving the memory of male mice and determine the solvent that has the best effectiveness. This study used male mice (Mus musculus) as test animals divided into eight groups, namely negative control, positive control, 96% ethanol extract at doses of 50, 100, and 200 mg/kgBW, and ethyl acetate extract at doses of 50, 100, and 200 mg/kgBW. Mice were induced using 10% ethanol, then tested using the Radial Arm Maze method based on the parameters of Working Memory Error (WME), Reference Memory Error (RME), and Time Latency. The results showed that administration of avocado leaf extract with both solvent variations was able to improve the memory of mice as indicated by a decrease in WME, RME, and Time Latency values. Ethyl acetate extract at a dose of 200 mg/kgBW provided the best effectiveness. This study demonstrates that variations in extraction solvents influence the biological effectiveness of avocado leaf extract, suggesting that ethyl acetate extract has the potential to be developed as a natural agent to support cognitive function.