Abstract
Abstract
Huntington’s disease (HD) is a fatal, neurodegenerative disorder driven by a CAG trinucleotide repeat expansion in the
huntingtin
(
HTT
) gene, resulting in neuronal toxicity and brain atrophy, initially in the most vulnerable brain region striatum and expanding to the cortex and other brain regions along disease progression. While cell-autonomous mechanisms within neurons have been extensively characterized, how brain waste clearance pathways at the brain’s borders change during disease progression remains poorly understood. In this study, we investigated the structural and functional adaptations of border-associated macrophages (BAMs) in the zQ175DN knock-in mouse model of HD. Immunohistochemical analysis revealed an age-dependent, selective reduction in the CD206
+
Lyve1
+
subset of leptomeningeal BAMs in 16-month-old zQ175DN mice, which tightly correlated with a profound impairment in cerebrospinal fluid (CSF) flow velocity and perivascular influx. To determine whether BAMs actively drives this CSF flow, we selectively depleted these populations of macrophages in 10-month-old zQ175DN mice via intracerebroventricular (i.c.v.) injection of clodronate liposomes (CLO). Chemical ablation of BAMs significantly exacerbated global CSF dynamic impairment, indicted by decreasing CSF fluorescent tracer distribution across perivascular parenchymal channels. Crucially, this CSF flow failure resulted in a marked accumulation of extranuclear mutant huntingtin (mHTT) aggregates in the zQ175DN HD mouse brain, while intranuclear inclusions were not significantly altered. Furthermore, BAMs depletion did not alter microglial or astrocytic gliosis in the brain, demonstrating that the exacerbation of mHTT load is a byproduct of brain waste clearance dysfunction. Together, our results indicate that BAMs are non-redundant regulators of CSF flow dynamics at the brain borders, offering a unique non-neuronal therapeutic target to combat HD.
Research Highlights
Age-dependent reduction of specific CD206
+
Lyve1
+
border macrophages (BAMs) occurs in HD mice.
Ablation of BAMs significantly restricts CSF flow in HD mice.
BAMs depletion selectively exacerbates extranuclear mHTT accumulation in HD mice.
mHTT clearance failure induced by BAMs depletion occurs independently of gliosis