FlyAnatoView: Visualization of Drosophila Single-cell RNA-seq Datasets on Anatomographs

ABSTRACT Single-cell RNA sequencing (scRNA-seq) is a powerful approach for measuring the transcriptomic landscape at single-cell resolution. However, while scRNA-seq data reveals what genes each cell expresses, they do not indicate where that cell was located within the tissue or organ, anatomically. We developed FlyAnatoView ( https://www.flyrnai.org/tools/rna_seq_base/web/svg_files/ ), a resource that projects scRNA-seq data onto anatomical diagrams, restoring this missing spatial context.