Abstract
Abstract
Myelin supports rapid and efficient neural communication and is implicated in brain development, learning, aging, and neurological disease. Although numerous magnetic resonance imaging (MRI) approaches have been proposed to characterize myelin in vivo, they have rarely been evaluated together within the same participants and across repeated acquisitions. To address these gaps, we introduce the Myelin Imaging Reliability and ReprOducibility Resource (MIRROR), an openly available multimodal test– retest MRI dataset of diffusion MRI (dMRI), inhomogeneous magnetization transfer imaging (ihMT), quantitative susceptibility mapping (QSM), MP2RAGE, T
1
w/T
2
w ratio mapping, and transverse relaxometry in 22 healthy adults. We evaluated gray–white matter differentiation, intermetric correspondence, test–retest reliability, and participant specificity across 27 myelin-sensitive metrics. Gray–white matter differentiation was strong for dMRI derivatives, MP2RAGE-derived R
1
, T
1
w/T
2
w, and ihMT, while being substantially weaker for QSM measures. Intermetric spatial correlations were generally strongest within acquisition or modeling families. Test–retest reliability was generally high, particularly for dMRI-derived metrics, but was more variable among QSM-, ihMT-, and R
2
*-derived measures. Regional spatial profiles were also highly participant-specific, with discriminability approaching its upper bound for most metrics. All imaging data, derived maps, and processing code are openly released. MIRROR provides a benchmark for characterizing the measurement properties and practical tradeoffs of scalable myelin-sensitive MRI methods, supporting principled acquisition selection and accelerating methodological development and validation.