Abstract
Abstract
Ion-pairs are central charged motifs that control the biological function of several proteins. Yet, the physical principles linked to the formation of ion-pairs during protein folding remain poorly understood. Here, we show that buried ion-pairs of
de novo
-designed helical bundle proteins reshape the folding pathways by introducing kinetic barriers that are overcome by a hydrophobic collapse linked to packing of the protein core. By combining protein design with co-translational folding studies, nuclear magnetic resonance (NMR) spectroscopy, and molecular dynamics simulations, we show that introduction of a buried ion-pair leads to a shifted folding transition that balances desolvation and electrostatic interactions within the protein and the bulk solvent. Moreover, we find that the folding pathway is sensitive to modifications in non-polar interactions and motifs that electrostatically shield the ion-pair from its surroundings. Our combined findings provide a blueprint for understanding how functional motifs affect protein folding and how mutation of charged networks alter the stability of proteins.
Significance Statement
Despite significant progress in machine learning-based structure prediction, we still lack a clear understanding of the folding pathways that determine how proteins fold into their exact three-dimensional structure. Here, we show that introduction of a buried ion-pair reshapes the folding pathway of
de novo
-designed protein
Maquettes
. By combining cotranslocation folding experiments of these proteins trapped within the ribosome exit tunnel with chemical unfolding experiments using nuclear magnetic resonance spectroscopy and atomistic simulations, we show that the ion-pair introduces a folding barrier linked to formation of the protein core. On a general level, our findings highlight how charged networks balance electrostatic and solvation forces in proteins, and how mutations can alter their folding landscape.