Abstract
Background and Objective: Farmers often rely on experience rather than quantitative indicators to determine seedling quality, resulting in inefficiencies in resource allocation. This study aimed to (1) compare seedling vigor and growth characteristics between open-pollinated (OP) and F1 hybrid cucumber seed types, and (2) develop a non-destructive predictive model for seedling vigor estimation based on digital image analysis.Methodology: Seedling images were acquired under controlled LED lighting using a 5 MP digital camera positioned 30 cm above 14-day-old seedlings. A YOLOv8-based object detection model was applied to detect and isolate true leaf regions, from which pixel count and RGB color values were extracted as input features for a multiple linear regression model to predict the seedling vigor index (SVI).Main Results: The YOLOv8 object detection model achieved a mean average precision (mAP@0.5) of 96.00%, precision of 93.40%, and recall of 94.40% in detecting true leaves. Multiple linear regression analysis was conducted using the Scikit-learn library in Python. Scikit-learn provides regression-based machine learning algorithms, including multiple linear regression. The equation was then applied to the training and test sets, using the pixel values of true leaves as the independent variable and SVI as the dependent variable. Using multiple regression analysis, the trained model generated the equation SVI = -2.27 + (2.84 × 10-5 × pixel) + (3.48 × 10-5 × R) + (-7.49 × 10-2 × G) + (2.06 × 10-1 × B), R2 = 0.57 and RMSE = 1.10. The model achieved the test set, r = 0.79 and RMSE = 1.34. The model performance showed a correlation coefficient of 0.85 for OP data and 0.91 for F1 hybrid data.Conclusions: The results demonstrate that digital image processing combined with object detection provides a non-destructive and effective approach to estimate seedling vigor quality. The predictive models developed from OP and F1 hybrid datasets indicate potential application in precision agriculture for automated seedling quality assessment and transplanting decision support.