Abstract
Efficient functioning of the prokaryotic translational system depends on a steady supply of aminoacylated tRNAs to be delivered to translating ribosomes via ternary complex. As such, tRNA synthetases play a crucial role in maintaining efficient and accurate translation in the cell, as they are responsible for aminoacylating the correct amino acid to its corresponding tRNA. Moreover, the kinetic rate at which they perform this reaction will dictate the overall rate of supply of aminoacylated tRNAs to the ribosome and will have consequences for the average translational speed of ribosomes in the cell. In this work, I develop an empirical kinetic model for the 20 aminoacyl tRNA synthetase enzymes in
E. coli
enabling the study of the effects of tRNA charging dynamics on translational efficiency. The model is parametrised based on
in vitro
experimental measurements of substrate
K
m
and
k
cat
values for both pyrophosphate exchange and aminoacylation. The model also reproduces the burst kinetics observed in class I enzymes and the transfer rates measured in single turnover experiments. Stochastic simulation of
in vivo
translation shows the kinetic model is able to support the tRNA charging demand resulting from translation in exponentially growing
E. coli
cells at a variety of different doubling times. This work provides a basis for the theoretical study of the amino acid starvation and the stringent response, as well as the complex behaviour of tRNA charging and translational dynamics in response to cellular stresses.